《植物生理学报》 2017, 53(2): 227-233

美国流苏离体胚的组织培养与快速繁殖

吴秀燕, 张鸽香^*

南京林业大学风景园林学院, 南京210037

通信作者：张鸽香；E-mail: nld_zhang@njfu.com.cn

摘　要：

以美国流苏成熟种胚为材料, 探讨不同培养基及植物生长调节剂对其萌发和植株再生的影响, 并对无菌苗茎段和叶片进行诱导增殖和生根研究。结果表明: 胚萌发适宜培养基为WPM+1.0 mg·L^-1 6-BA, 萌发率达96.3%, 成苗率73.9%, 平均苗高6.51 cm。茎段诱导不定芽适宜培养基为WPM+2.0 mg·L^-1 6-BA+0.2 mg·L^-1 NAA, 诱导率达到86.8%, 繁殖系数为5.77。叶片愈伤组织诱导适宜培养基为WPM+3.0 mg·L^-1 6-BA+0.5 mg·L^-1 2,4-D, 诱导率达到45.0%。适宜的生根培养基为1/2MS+1.0 mg·L^-1 IBA+0.5 mg·L^-1 NAA, 生根率达到89.8%, 平均主根长为3.03 cm。移栽率成活率为87%。

关键词：美国流苏; 离体胚; 组织培养; 快速繁殖

收稿：2016-11-22   修定：2017-01-04

资助：国家林业局“948”项目(2014-4-17)和江苏省高校学科建设工程资助项目(PAPD)。

Study on embryo culture and rapid propagation in vitro of Chionanthus virginicus

WU Xiu-Yan, ZHANG Ge-Xiang^*

College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China

Corresponding author: ZHANG Ge-Xiang; E-mail: nld_zhang@njfu.com.cn

Abstract:

Using the embryo of Chionanthus virginicus as the material, the effects of different concentrations and combinations of plant growth regulators on the germination and plantlet regeneration, stem segments and leaves induction of proliferation and rooting were discussed. The results showed that the suitable embryo rooting medium was WPM+1.0 mg∙L^-1 6-BA, the germination rate was 96.3%, the seedling rate was 73.9%, embryo germination average height was 6.51 cm. the suitable medium for stem segments inducing adventitious buds was WPM+2.0 mg∙L^-1 6-BA+0.2 mg∙L^-1 NAA, The induction rate was 86.8%, the propagation coefficient was 5.77. The suitable medium for induction of callus from leaf explants was WPM+3.0 mg∙L^-1 6-BA+0.5 mg∙L^-1 2,4-D, the induction rate was 45.0%, and the suitable rooting medium was 1/2MS+1.0 mg·L^-1 IBA+0.5 mg·L^-1 NAA, the rooting rate was 89.8%, and the average root length was 3.03 cm. And the survival rate was 87%.

Key words: Chionanthus virginicus; embryo; tissue culture; rapid propagation